Back to article: Antagonism between salicylate and the cAMP signal controls yeast cell survival and growth recovery from quiescence

FIGURE 1: Limited salicylate effects on proliferation of active yeast populations. (A) Mild transient inhibition of yeast exponential growth with salicylate. An exponentially growing culture of wt yeast cells (black) was supplied (red arrow) with 3 mM salicylate (red). The cell number per ml (upper graph) and the Optical Density (OD) at 600nm (lower graph) were monitored over time. The Log10 of each value was graphically plotted versus time. After a transient and modest growth inhibition due the drug the culture Doubling Time (DT) returned similar to that of untreated control. DTs from the cell number increases: 1.66 (control) and 1.69 (SA) hours. DTs from OD600 values: 2.1 (control) and 2.2 (SA) hours. DTs were calculated in late log phase (last 5 points), so are expected to be longer if derived from O.D.600. (B) Unaltered lag phase before growth recovery of chronologically young yeast populations supplied with salicylate. wt yeast cells were re-inoculated into fresh medium after 2 days (upper panel) or 4 days (lower panel) spent into stationary phase. OD600nm values were monitored in control untreated cells (black) and cells supplied with 3 mM salicylate (red). Values of Means (±SEM) derived from three independent repeats (each performed in triplicate) were plotted against time.

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