FIGURE 1: Mic27 interacts genetically with Mic60 and deletion of Mic27 leads to altered cristae morphology. (A) Growth of WT, Δmic26, Δmic27, Δmic60, Δmic27/Δmic60, and Δmic26/Δmic60 strains were examined by serial dilutions on YPD (30°C) and YPEG medium (30 °C and 37°C). (B) Indicated strains were cultivated in YPEG, whole cells were chemically fixed and cryo-sectioned, and mitochondrial ultrastructure was determined by standard transmission electron microscopy. Size bars represent 100 nm. (C,D) Cryo-electron tomography of isolated wild type (C) and Δmic27 (D) mitochondria. Left: slice from a tomogram. Right: surface rendered representation of a crista indicated by red arrow in tomographic slice.