FIGURE 2: Translation initiation is inhibited by farnesol in a Gcn2p-independent manner in C. albicans and S. cerevisiae. Figure shows polysome analyses assessing the effect of farnesol on translation initiation in CAI4 (A) and gcn2∆ (B) strains of C. albicans, and the Σ1278b strain of S. cerevisiae (C). Strains were grown in YPD and various concentrations of farnesol were added as indicated for 15 min prior to extract preparation. Extracts were sedimented on 15-50% sucrose gradients and the absorbance at 254 nm was continuously measured. The position of 40S, 60S and 80S peaks are labelled and the direction of sedimentation is noted.