Back to article: Impact of histone H4K16 acetylation on the meiotic recombination checkpoint in Saccharomyces cerevisiae

FIGURE 4: Analysis of Mek1 activation and localization in ndt80-arrested cells.

(A) Western blot analysis of different Mek1 phosphorylation forms in ndt80-arrested cells after 24 h in meiosis. PGK is shown as a loading control. Strains are DP424 (wild type), DP428 (zip1), DP996 (zip1 H4-K16R) and DP1002 (zip1 H4-K16Q). Two independent clones of DP966 and DP1002 were analyzed.

(B) Representative images of checkpoint-induced Mek1-GFP foci in wild type (DP584), zip1 (DP582), zip1 H4-K16R (DP1089) and zip1 H4-K16Q (DP1090) ndt80-arrested cells after 24 h in meiosis.

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