Back to article: Impact of histone H4K16 acetylation on the meiotic recombination checkpoint in Saccharomyces cerevisiae

FIGURE 7: DOT1 overexpression partially restores the meiotic checkpoint in H4K16ac-deficient mutants.

(A) Western blot analysis of H3K79 methylation species in vegetative (T=0h) and meiotic cells (T=20h). Total H3 and PGK were used as loading controls.

(B) Quantification of relative levels of the H3K79 methylation forms at T=0 from the blots shown in (A). Total H3 signal was used for normalization.

(C) Time course of meiotic nuclear divisions; the percentage of cells with two or more nuclei is presented. Strains are DP422 (zip1), DP995 (zip1 H4-K16R) and DP1001 (zip1 H4-K16Q), transformed either with an empty vector or with the high-copy pSS63 DOT1 overexpression plasmid (DOT1-OE).

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