Back to article: Impact of histone H4K16 acetylation on the meiotic recombination checkpoint in Saccharomyces cerevisiae

FIGURE 9: Sir2 excludes H4K16ac from the rDNA region.

Immunofluorescence of meiotic chromosome spreads from wild type (BR2495), sir2 (DP262), zip1 (DP1123) and zip1 sir2 (DP1124), stained with DAPI (red) as well as with anti-H4K16ac (green) and anti-Nsr1 (blue) antibodies. The arrows point to the rDNA region identified by Nsr1 staining. Representative nuclei are shown. Spreads were prepared after 16 h of meiotic induction for BR2495 and DP262 and 24 h for DP1123 and DP1124.

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