FIGURE 5: Normal metacyclogenesis in absence of polyP in L. major. Metacyclic promastigotes of WT and vtc4^– L. major clones were isolated by PNA assay (A) or by Ficoll centrifugation gradient (B). Results of a pool of 2 independent experiments were expressed as mean ± SD. Statistical significance was assessed by Student’s t-test (A and B); ns: non-significant.