Back to article: Mutational analysis of fructose-1,6-bis-phosphatase FBP1 indicates partially independent functions in gluconeogenesis and sensitivity to genotoxic stress
FIGURE 4: Drop-tests on Non-fermentable (SDEG) and MMS-containing agar-media.
(A) Drop-test on SDEG non-fermentable medium. Wt and mutated FBP1 cassettes were introduced into BY4741 fbp1∆ cells lacking endogenous FBP1, the resulting strains were grown in liquid SD-Ura medium, diluted and spotted on SDEG, same suspensions were spotted on SD-Ura agar as a control. Agar-plates were incubated for 48 h at 30°.
(B and C) Drop tests on SD-Ura agar with 0.01% MMS. Mutants and wt cassettes were introduced into both wt (B) and ∆fbp1 (C) BY4741 cells. The resulting strains were grown in SD-Ura and then diluted (1:5) and spotted on freshly prepared SD-Ura containing 0.01%, and on SD-Ura as non-treated control. Agar plates were incubated for 48 h at 30°C.
(D and E) Drop tests on SD-Ura agar containing 0.015% and 0.0175% MMS. Plates were incubated for 72 h at 30°C.
(F to I) Quantifications of the spot-intensities of the drop tests on SD-Ura 0.01% MMS second and third spots (OD600: 2 and 0.4 respectively) and 0.0175% first and second spots (OD600: 10 and 2 respectively). Featured results are taken from three biologically independent replicates, (error-bars: SD; N = 3).