FIGURE 5: Neither trehalose accumulation nor TPS1 activity accounted for cellular memory. (A) The indicated cells were extracted and assayed for trehalose levels using an enzymatic assay. Error bars represent 1 standard error of the mean from 3 biological replicates. (B) These same cells were diluted into YPD media and trehalose levels of the resulting cultured cells were determined at the indicated timepoints. Error bars represent 1 standard error of the mean from 3 biological replicates (C) H₂O₂ stress resistance spot assay of 12 hours galactose starvation (Starved) and mid-log cells (Naive) of WT and tps1Δ are shown. NS represents cells that were not stressed with peroxide. (D) 10-fold serial dilution spot assay of WT and tps1Δ on either SC-Galactose plates (Galactose) or SC-Glucose (Glucose) plates.