Figure 2: Rlp24 functions as a placeholder factor for the L24 r-protein. (A) Position of Rlp24 (red) in the early pre-60S r-particles purified with Nog2-TAP (PDB ID: 3JCT; [40]). (B) Position of L24 r-protein (red) in the mature 60S r-subunit (PDB ID: 3U5I, 3U5H; [44]). Particles are viewed from the subunit interface. For orientation, the positions of the 5S rRNA (yellow) and the L23 r-protein (royal blue) have been highlighted. The ITS2 foot has also labelled in A. Note that the last ca. 50 amino acids from the C-terminal part of Rlp24 and ca. 20 amino acids from the C-terminal end of L24 could not be modelled in the respective structures. The rest of rRNAs are coloured in pale blue and the rest of r-proteins and/or factors in light cornflower blue.

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