Figure 3: Rlp7 is not the placeholder factor for the assembly of L7 r-protein. (A) Position of Rlp7 (red) and L7 (purple) in the early pre-60S r-particles purified with Nog2-TAP (PDB ID: 3JCT; [40]). (B) Position of L7 r-protein (purple) in the mature 60S r-subunit (PDB ID: 3U5I, 3U5H; [44]). Particles are viewed from the solvent side. For orientation, the positions of the 5S rRNA (yellow), 5.8S rRNA (gold) and the L9 r-protein (royal blue) have been highlighted. The ITS2 foot has also labelled in A. Note that L7 is found at its final assembly position within Nog2-TAP pre-60S r-particles. The first ca. 20 amino acids of both proteins could not be modelled in either structure. The rest of rRNAs are coloured in pale blue and the rest of r-proteins and/or factors in light cornflower blue.

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