Back to article: Placeholder factors in ribosome biogenesis: please, pave my way

Figure 3: Rlp7 is not the placeholder factor for the assembly of L7 r-protein. (A) Position of Rlp7 (red) and L7 (purple) in the early pre-60S r-particles purified with Nog2-TAP (PDB ID: 3JCT; [40]). (B) Position of L7 r-protein (purple) in the mature 60S r-subunit (PDB ID: 3U5I, 3U5H; [44]). Particles are viewed from the solvent side. For orientation, the positions of the 5S rRNA (yellow), 5.8S rRNA (gold) and the L9 r-protein (royal blue) have been highlighted. The ITS2 foot has also labelled in A. Note that L7 is found at its final assembly position within Nog2-TAP pre-60S r-particles. The first ca. 20 amino acids of both proteins could not be modelled in either structure. The rest of rRNAs are coloured in pale blue and the rest of r-proteins and/or factors in light cornflower blue.

40. Wu S, Tutuncuoglu B, Yan K, Brown H, Zhang Y, Tan D, Gamalinda M, Yuan Y, Li Z, Jakovljevic J, Ma C, Lei J, Dong MQ, Woolford JL, Jr., Gao N (2016). Diverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomes. Nature 534(7605): 133-137.

44. Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M (2011). The structure of the eukaryotic ribosome at 3.0 Å resolution. Science 334(6062): 1524-1529.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.