Figure 6: Interactions at the polypeptide exit tunnel of pre-60S ribosomal particles. (A) Position of Nog1 (red) and Arx1 (dark gold) in the early pre-60S r-particle purified with Nog2-TAP (PDB ID: 3JCT; [40]). Note that the C-terminal part of Nog1 enters the tunnel. The location of Mrt4 (cyan) is also shown. (B) Position of Rei1 (red) and Arx1 (dark gold) in a reconstituted 60S r-subunit (PDB ID: 5APN; [68]). Note that only the middle part (141-261) and the C-terminal end (300-393) of the protein is visualised. As above, the C-terminal part of Rei1 enters the tunnel. The location of P0 (cyan) is also shown. (C) Position of Reh1 (red) in the late/cytoplasmic pre-60S r-particles purified with Nmd3-TAP (PDB ID: 5H4P; [43]). Note that only the region of the protein occluding the tunnel could be visualised (amino acids 377-432 of 432). Particles are viewed from the solvent side. For orientation, the locations of L5 (yellow) and L39 (blue) have been highlighted. The rest of rRNAs are coloured in pale blue and the rest of r-proteins and/or factors in light cornflower blue.

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43. Ma C, Wu S, Li N, Chen Y, Yan K, Li Z, Zheng L, Lei J, Woolford JL, Jr., Gao N (2017). Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1. Nat Struct Mol Biol 24(3): 214-220. https://doi.org/10.1038/nsmb.3364

68. Greber BJ, Gerhardy S, Leitner A, Leibundgut M, Salem M, Boehringer D, Leulliot N, Aebersold R, Panse VG, Ban N (2016). Insertion of the biogenesis factor Rei1 probes the ribosomal tunnel during 60S maturation. Cell 164(1-2): 91-102. https://doi.org/10.1016/j.cell.2015.11.027