Back to article: Sulfur transfer and activation by ubiquitin-like modifier system Uba4•Urm1 link protein urmylation and tRNA thiolation in yeast

FIGURE 2: Overexpression of tRNAs subject to Urm1-dependent U34 thiolation fails to suppress loss of Ahp1 urmylation at 39°C.

(A) Urmylation of Ahp1 is suppressed at 39°C. An urm1∆ strain expressing TAP-URM1 was grown to logarithmic growth phase and split into two cultures prior to cultivation at 30°C or 39°C for three hours (h). Ahp1 urmylation analysis involved anti-TAP-based EMSA and immune blots with anti-Ahp1 to detect non-conjugated (free) Ahp1. Protein loading was controlled with anti-Cdc19 antibodies.

(B) tRNAGln, tRNALys and tRNAGlu (tQKE) overexpression cannot rescue defective Ahp1 urmylation at 39°C. Except for tQKE overexpression from multi-copy vector pQKE (Table S3), cell growth at 30°C or 39°C and urmylation analysis were as described in (A). Arrows (A, B) indicate the positions of non-conjugated (free) forms of TAP-Urm1 and Ahp1, as well as TAP-Urm1 conjugated to Ahp1 and loading control Cdc19.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.