Back to article: Thiol trapping and metabolic redistribution of sulfur metabolites enable cells to overcome cysteine overload


FIGURE 6. Gene expression changes due to cysteine or cystine addition to cells.

(A) Gene ontology based analysis (by process), illustrating induced genes that are dramatically overrepresented (by process) upon cysteine addition.

(B) A mapping of highly induced genes onto the ornithine/arginine biosynthesis pathway. The heat-map shows (log2) fold change in transcripts induced.

(C) A schematic of the metabolic pathways used to make polyamines, spermidine and spermine, emphasizing the use of SAM (S-Adenosyl L-methionine) and ornithine in this process.

(D) and (E) relative changes in spermidine amounts over time, upon the addition of cysteine (D) or cystine (E) to cells expressing YCT1 or CgCYN1 respectively.