Back to article: Trehalose-6-phosphate promotes fermentation and glucose repression in Saccharomyces cerevisiae

FIGURE 3: Resumption of growth of S. cerevisiae tps1 mutant transformed with TPS1 from different organism on YN glucose media in microtiter plate. (A) Complementation of sctps1 with TPS1 homologs from yeast species. (B) Complementation of Sctps1 with TPSD1 homologs from P. pastoris, H. polymorpha and the filamentous fungi A. niger and M. grisea. (C) Complementation of sctps1 with TPS1 homologs from bacteria, plants and drosophila. The experimental design was as in Figure 2 except that the yeast cells were inoculated in 250 µL of YN containing glu-cose at 2% (w/v). Results shown are the mean of two independent experiments. For the sake of clarity, standard devia-tion bars have been withdrawn, but overall the SD was in the range of 10 – 15 %.

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