Back to article: Trehalose-6-phosphate promotes fermentation and glucose repression in Saccharomyces cerevisiae

TABLE 2. Activity of Trehalose 6-P synthase (Tps1) and levels of trehalose in the S. cerevisiae tps1 mutant transformed by TPS1 of various origins.

The activity of T6P synthase was measured at 42°C as described in Material and Methods. Results are the mean ± SD of three independent assays. Trehalose was determined in stationary phase approximatively 12 h after glucose exhaustion from the medium, at a time when trehalose content is about to reach its maximum and stabilizes for several hours before consumption [68].

68. Francois J, Neves MJ, Hers HG (1991). The control of trehalose biosynthesis in Saccharomyces cerevisiae: evidence for a catabolite inactivation and repression of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase. Yeast 7(6): 575-587.

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