Research Articles – Page 4

Research, Research Articles

Promoter methylation and increased expression of PD-L1 in patients with active tuberculosis

Yen-Han Tseng^1,2, Sheng-Wei Pan^1,2,3, Jhong-Ru Huang^2,4, Chang-Ching Lee¹, Jung-Jyh Hung^2,5, Po-Kuei Hsu^2,5, Nien-Jung Chen⁶, Wei-Juin Su^2,7, Yuh-Min Chen^1,2 and Jia-Yih Feng^1,2,8

The PD-1/PD-L1 pathway plays a pivotal role in T cell activity and is involved in the pathophysiology of tuberculosis. Here we show that PD-L1 expression is increased in patients with active tuberculosis and is correlated with treatment outcomes.

Quantiﬁcation methods of Candida albicans are independent irrespective of fungal morphology

Amanda B Soares¹, Maria C de Albuquerque¹, Leticia M Rosa¹, Marlise I Klein ², Ana C Paravina¹, Paula A Barbugli¹, Livia N Dovigo³ and Ewerton G de O Mima¹

Our study demonstrated that the quantification methods of C. albicans (cells/mL, CFU/mL, and vPCR) did not agree, regardless of the fungal morphology/growth, even though a significant and strong correlation is observed.

Pathogenic Escherichia coli change the adhesion between neutrophils and endotheliocytes in the experimental bacteremia model

Svetlana N Pleskova^1,2,*, Nikolay A Bezrukov¹, Sergey Z Bobyk¹, Ekaterina N Gorshkova¹ and Dimitri V Novikov³

In this work, we have demonstrated that in the model of experimental septicemia there is a disruption of adhesion contacts between neutrophils and endothelial cells, manifested by a decrease in adhesion force and work upon exposure to E. coli.

Arsenite treatment induces Hsp90 aggregates distinct from conventional stress granules in ﬁssion yeast

Naofumi Tomimoto^a, Teruaki Takasaki^a and Reiko Sugiura

Given the conserved role of Hsp90 as a molecular chaperone protein, our findings presented in this study may suggest a novel type of arsenite-induced biological condensates, wherein Hsp90 plays a key role in maintaining its integrity.

Unresolved mystery of cyclic nucleotide second messengers, periplasmic acid phosphatases and bacterial natural competence

Kristina Kronborg and Yong Everett Zhang

In this study we aimed to identify the promotors responsible for the expression of the non-specific acid phosphatase AphA during different starvation conditions, to confirm the requirement of the cAMP-dependent CRP regulon for aphA expression, and to finally identify regulators of its expression.

Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U- ExM protocols

Clirim Jetishi^1,2,a,Erina A. Balmer^1,2,a, Bianca M. Berger^1,2,a, Carmen Faso^1,3,4and Torsten Ochsenreiter¹

Understanding cellular ultrastructure is tightly bound to microscopic resolution and the ability to identify individual components at that resolution. In this study we demonstrate mostly isotropic 4.5-fold expansion of several different compartments in Giardia cells and present an optimised, shortened, and modular protocol that can be swiftly adjusted to the investigators needs.

Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome

Anusha Chaudhuri^1,#, Soumita Paul^2,#, Mayukh Banerjea² and Biswadip Das²

In this investigation, we unveiled a novel functional role of the major nuclear 3′→5′ exoribonuclease, Rrp6p, and its cofactor Rrp47p in the degradation of polyadenylated versions of several mature sncRNAs, including 5S, 5.8S rRNAs, all sn- and some select snoRNAs in the baker’s yeast S. cerevisiae.

Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches

Svenja Braam¹, Farida Tripodi², Linnea Österberg^1,3, Sebastian Persson¹, Niek Welkenhuysen¹, Paola Coccetti² and Marija Cvijovic¹

In this work we set out to explore the relationship between the subcellular localization and regulation of kinases in the context of carbon source signaling. The data presented in this paper reinforce the notion that not only the activation/inactivation of kinases but also their subcellular localization and that of their targets influence fate decisions in response to environmental changes.

A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae

Katrin Hieronimus^1,2,#, Tabea Donauer^1,2,#, Jonas Klein^1,#, Bastian Hinkel^1,#, Julia Vanessa Spänle^1,#, Anna Probst^1,#, Justus Niemeyer^1,#, Salina Kibrom¹, Anna Maria Kiefer¹, Luzia Schneider², Britta Husemann², Eileen Bischoff², Sophie Möhring², Nicolas Bayer¹, Dorothée Klein¹, Adrian Engels¹, Benjamin Gustav Ziehmer², Julian Stieß³, Pavlo Moroka¹, Michael Schroda¹, and Marcel Deponte²

Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs. We established a MoClo toolkit and exemplified its application for the production of recombinant proteins in L. tarentolae.

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RidA proteins contribute to ﬁtness of S. enterica and E. coli by reducing 2AA stress and moderating ﬂux to isoleucine biosynthesis

October 4, 2024

This study solidifies the established role of RidA in removing 2AA, while also presenting evidence for a role of RidA in enhancing ﬂux towards isoleucine biosynthesis in E. coli. Overall, these data emphasize that metabolic networks can generate distinct responses to perturbation, even when the individual components are conserved.

Fecal gelatinase does not predict mortality in patients with alcohol-associated hepatitis

August 26, 2024

This study aimed to investigate the significance of fecal gelatinase on clinical outcomes in patients with alcohol-associated hepatitis. In conclusion, in our cohort, fecal gelatinase does not predict mortality and does not indicate higher disease severity in patients with alcohol-associated hepatitis.