Research, Research Articles

Overcoming phagocytosis resistance of hypervirulent <i>Klebsiella pneumoniae</i> by directly targeting capsules

Overcoming phagocytosis resistance of hypervirulent Klebsiella pneumoniae by directly targeting capsules

Shogo Tsubaki1, Touya Toyomoto1, Rika Tanaka2,3, Jin Imai4,5, Juntaro Matsuzaki6,7, Katsuto Hozumi2 and Hitoshi Tsugawa1,5*

This study highlights a promising strategy for disarming hypervirulent K. pneumoniae by directly targeting its key virulence factors and provides novel insights into antibacterial therapeutic approaches against this clinically significant pathogen.

VapA/Scs2 sustains polarized growth in <i>Aspergillus nidulans</i> by maintaining AP-2-mediated apical endocytosis

VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis

Xenia Georgiou1, Sofia Politi1, Sotiris Amillis1 and George Diallinas1,2,*

To explore the functional significance of ER–PM contact sites in filamentous fungi, we identified and genetically characterized all Aspergillus nidulans proteins homologous to Snc2/VAP, Ist2, or tricalbins.

Genetic make-up and regulation of the L-lysine biosynthesis pathway in <i>Vibrio natriegens</i>

Genetic make-up and regulation of the L-lysine biosynthesis pathway in Vibrio natriegens

Elly Straube1, Johannes Radde1, Thi Van Anh Tran1, Negin Keihani Yazdi1, Rubén Crespo Blanco1, Ha Thanh Le2, Cláudio J.R. Frazão1 and Thomas Walther1,*

This study analysed the make-up and regulation of the biosynthetic pathway for L-lysine and related L-aspartate family amino acids (AFAAs) in Vibrio natriegens DSM759 to provide a comprehensive basis for future metabolic engineering endeavours aiming at developing this strain into an amino acid overproducer.

Regulation of extracellular vesicles for protein secretion in <i>Aspergillus nidulans</i>

Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans

Rebekkah E. Pope1, Patrick Ballmann2, Lisa Whitworth3 and Rolf A. Prade1,*

This study reveals that Aspergillus nidulans boosts extracellular vesicle production when ER-trafficked enzymes are induced, uncovering how fungi remodel their secretome through vesicle-mediated secretion to adapt to changing environments and biofilm formation.

Transcriptomic response to different heme sources in <i>Trypanosoma cruzi</i> epimastigotes

Transcriptomic response to different heme sources in Trypanosoma cruzi epimastigotes

Evelyn Tevere1,a, María G. Mediavilla1,a, Cecilia B. Di Capua1, Marcelo L. Merli1, Carlos Robello2,3, Luisa Berná2,4 and Julia A. Cricco

This study uncovers how the Chagas disease parasite adapts to changes in heme, an essential molecule for its survival, providing transcriptional clues to heme metabolism and identifying a previously unreported heme-binding protein in T. cruzi.

Sir2 regulates selective autophagy in stationary-phase yeast cells

Ji-In Ryua, Juhye Junga, and Jeong-Yoon Kim

This study establishes Sir2 as a previously unrecognized regulator of selective autophagy during the stationary phase and highlight how cells dynamically control organelle degradation.

Luminal acetylation of microtubules is not essential for <i>Plasmodium berghei</i> and <i>Toxoplasma gondii</i> survival

Luminal acetylation of microtubules is not essential for Plasmodium berghei and Toxoplasma gondii survival

Thrishla Kumar1,a, Katharina Röver2,a, Johannes F. Stortz3,a, Annika M. Binder2,a, Benjamin Spreng2, Madlen Konert2, Markus Meissner1, Friedrich Frischknecht2,4 and Elena Jimenez-Ruiz1,*

Acetylation of α-tubulin at lysine 40 is not essential for cytoskeletal stability in Plasmodium berghei or Toxoplasma gondii, suggesting redundancy and plasticity in microtubule regulation in these parasites.

The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in <i>Saccharomyces cerevisiae</i>

The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in Saccharomyces cerevisiae

Angela Cirigliano1,a, Antonia Amelina2,a, Elena Passarini2, Alessandra Ricelli1, Nicole Balasco1, Mattia Mori3, Bruno Botta4, Maria Egle De Stefano2,5, Claudio Papotto6, Claudia Guerriero2, Ada Maria Tata2,5 and Teresa Rinaldi2,*

S. cerevisiae is a model to study human GPCRs. N-8-Iper, active against glioblastoma via M2 receptor, causes mitochondrial damage in yeast by binding Ste2, highlighting evolutionary conservation of GPCRs.

The core genetic drivers of chronological aging in yeast are universal regulators of longevity

Erika Cruz-Bonilla1, Sergio E. Campos2, Soledad Funes3, Cei Abreu-Goodger4 and Alexander DeLuna1,2,*

This study provides an integrated view of the core genetic landscape underlying aging in yeast, highlighting the value of the chronological lifespan paradigm for investigating conserved mechanisms of aging.

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Overcoming phagocytosis resistance of hypervirulent Klebsiella pneumoniae by directly targeting capsules

February 16, 2026

This study highlights a promising strategy for disarming hypervirulent K. pneumoniae by directly targeting its key virulence factors and provides novel insights into antibacterial therapeutic approaches against this clinically significant pathogen.

VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis

February 5, 2026

To explore the functional significance of ER–PM contact sites in filamentous fungi, we identified and genetically characterized all Aspergillus nidulans proteins homologous to Snc2/VAP, Ist2, or tricalbins.

Genetic make-up and regulation of the L-lysine biosynthesis pathway in Vibrio natriegens

February 3, 2026

This study analysed the make-up and regulation of the biosynthetic pathway for L-lysine and related L-aspartate family amino acids (AFAAs) in Vibrio natriegens DSM759 to provide a comprehensive basis for future metabolic engineering endeavours aiming at developing this strain into an amino acid overproducer.

Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans

January 28, 2026

This study reveals that Aspergillus nidulans boosts extracellular vesicle production when ER-trafficked enzymes are induced, uncovering how fungi remodel their secretome through vesicle-mediated secretion to adapt to changing environments and biofilm formation.

Transcriptomic response to different heme sources in Trypanosoma cruzi epimastigotes

January 23, 2026

This study uncovers how the Chagas disease parasite adapts to changes in heme, an essential molecule for its survival, providing transcriptional clues to heme metabolism and identifying a previously unreported heme-binding protein in T. cruzi.

Sir2 regulates selective autophagy in stationary-phase yeast cells

January 21, 2026

This study establishes Sir2 as a previously unrecognized regulator of selective autophagy during the stationary phase and highlight how cells dynamically control organelle degradation.

Luminal acetylation of microtubules is not essential for Plasmodium berghei and Toxoplasma gondii survival

December 17, 2025

Acetylation of α-tubulin at lysine 40 is not essential for cytoskeletal stability in Plasmodium berghei or Toxoplasma gondii, suggesting redundancy and plasticity in microtubule regulation in these parasites.

The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in Saccharomyces cerevisiae

December 12, 2025

S. cerevisiae is a model to study human GPCRs. N-8-Iper, active against glioblastoma via M2 receptor, causes mitochondrial damage in yeast by binding Ste2, highlighting evolutionary conservation of GPCRs.

The core genetic drivers of chronological aging in yeast are universal regulators of longevity

October 31, 2025

This study provides an integrated view of the core genetic landscape underlying aging in yeast, highlighting the value of the chronological lifespan paradigm for investigating conserved mechanisms of aging.

Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure –ERMES– is essential for Podospora anserina development

September 12, 2025

Eucaryotic cell functioning and development depend on the concerted activity of its organelles. In the model fungus Podospora anserina, sexual development involves a dynamic regulation of mitochondria, peroxisomes and the endoplasmic reticulum (ER), suggesting that their activity during this process is coordinated.

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