Research, Research Articles
Genomic epidemiology of carbapenemase-producing Klebsiella pneumoniae circulating in a Chilean tertiary-care hospital (2021–2022): Molecular characterization, resistance-virulence convergence, and clinical associations
Gustavo Araya1,2, Alhejandra Álvarez1, Carolina Arellano1, Rodrigo Bravo3, Abraham Gajardo4,5, Boris Barrera6, Francisco Silva6 and Roberto M. Vidal1
This study characterized carbapenem-resistant Kp (CR-Kp) strains isolated at the Hospital Clínico Universidad de Chile (HCUCH) 2021-2022 and explored associations with clinical characteristics.
Sugar-induced cell death (SICD) in Saccharomyces cerevisiae: insights into nitrogen-mediated rescue and apoptotic cell death pathways
Raveena Parbhudayal and Hai-Ping Cheng
This study examined mechanisms through which yeast sugar-induced cell death can be prevented. High concentrations of glucose induced a catastrophic response that was only rescued by highly preferred nitrogen sources and by preventing nuclear localization of specific cell death proteins.
TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene CLN3
Vijaykumar Pillalamarri1, Samuel W.M. Gatesy1, Amanda E. Grassel1, Lucienna Wolf1, Justin P. Whalley2 and David M. Mueller1,*
This study identifies conditions and genes that induce BTN1 expression in yeast. We show that BTN1 expression is regulated by translational control and by the mTOR1 pathway. An understanding of when and why BTN1 expression will aid in understanding the expression of CLN3, which may be helpful in the treatment of this devastating disease.
Metagenomic and microbiological analyses of historical manuscripts for bacterial community profiling and bacteria-related biodeterioration assessment
Esra Keles1,* and Ozge Celik2
By documenting both culturable and non-culturable taxa, this work provides a foundational dataset for understanding bacterial contributions to manuscript stability and offers a methodological framework for future research on biodeterioration dynamics in Islamic and global documentary heritage.
Overcoming phagocytosis resistance of hypervirulent Klebsiella pneumoniae by directly targeting capsules
Shogo Tsubaki1, Touya Toyomoto1, Rika Tanaka2,3, Jin Imai4,5, Juntaro Matsuzaki6,7, Katsuto Hozumi2 and Hitoshi Tsugawa1,5*
This study highlights a promising strategy for disarming hypervirulent K. pneumoniae by directly targeting its key virulence factors and provides novel insights into antibacterial therapeutic approaches against this clinically significant pathogen.
VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis
Xenia Georgiou1, Sofia Politi1, Sotiris Amillis1 and George Diallinas1,2,*
To explore the functional significance of ER–PM contact sites in filamentous fungi, we identified and genetically characterized all Aspergillus nidulans proteins homologous to Snc2/VAP, Ist2, or tricalbins.
Genetic make-up and regulation of the L-lysine biosynthesis pathway in Vibrio natriegens
Elly Straube1, Johannes Radde1, Thi Van Anh Tran1, Negin Keihani Yazdi1, Rubén Crespo Blanco1, Ha Thanh Le2, Cláudio J.R. Frazão1 and Thomas Walther1,*
This study analysed the make-up and regulation of the biosynthetic pathway for L-lysine and related L-aspartate family amino acids (AFAAs) in Vibrio natriegens DSM759 to provide a comprehensive basis for future metabolic engineering endeavours aiming at developing this strain into an amino acid overproducer.
Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans
Rebekkah E. Pope1, Patrick Ballmann2, Lisa Whitworth3 and Rolf A. Prade1,*
This study reveals that Aspergillus nidulans boosts extracellular vesicle production when ER-trafficked enzymes are induced, uncovering how fungi remodel their secretome through vesicle-mediated secretion to adapt to changing environments and biofilm formation.
Transcriptomic response to different heme sources in Trypanosoma cruzi epimastigotes
Evelyn Tevere1,a, María G. Mediavilla1,a, Cecilia B. Di Capua1, Marcelo L. Merli1, Carlos Robello2,3, Luisa Berná2,4 and Julia A. Cricco
This study uncovers how the Chagas disease parasite adapts to changes in heme, an essential molecule for its survival, providing transcriptional clues to heme metabolism and identifying a previously unreported heme-binding protein in T. cruzi.
Scp160 deletion suppresses TDP-43 aggregation and toxicity in Saccharomyces cerevisiae
October 8, 2026
Deletion of SCP160 or BFR1 reduces TDP-43 toxicity without lowering TDP-43 protein abundance and suppresses prominent TDP-43, Htt103Q, and VHL aggregation, while SCP160 deletion also shifts residual TDP-43 foci toward the ER.
The p53 DNA-Binding (Core) Domain can form a prion that seeds prion formation in full-length p53
October 2, 2026
In yeast the p53 core-domain forms a self-propagating prion that inactivates a reporter and converts untagged full-length p53 to a prion. R175H in the tagged chimera shows 18x higher prionization, linking it to p53 inactivation in human cancers.
Sorbic acid triggers rapid formation of proteasome storage granules in Saccharomyces cerevisiae
August 12, 2026
This study demonstrates that sorbic acid, a common food preservative, induces the fastest reported proteasome storage granule (PSG) formation in yeast within 30 min, highlighting differences from other PSG-inducing conditions like acetic acid stress.
A time-resolved high-throughput screening of fission yeast deletion mutants for oxidative stress resistance
July 22, 2026
By capturing dynamic trajectories rather than static outcomes, this study exposes hidden layers of growth under oxidative stress and identifies new genetic determinants of cellular resilience in fission yeast.
Clonal spread and environmental persistence of carbapenem-resistant high-risk Pseudomonas aeruginosa in critical-care units of a Chilean national referral center for burn and trauma patients (2022)
July 20, 2026
This study highlight the circulation of high-risk P. aeruginosa clones in Chile and underscore the importance of molecular epidemiology in guiding infection control, optimizing antimicrobial therapy, and mitigating the clinical and economic burden of CRPA.
The transcriptome of dendritic cells redraws the boundaries between pathogenicity and commensalism in yeast
July 7, 2026
Our immune system meets many fungi, but reacts strongly to only a few. The comparison of key fungal species showed that responses differ mainly in timing and not just genes, highlighting the need to rethink what makes fungi harmful vs. harmless.
Yippee-like protein Moh1 links gene expression to metabolism and selective stress resistance in Saccharomyces cerevisiae
June 29, 2026
This study demonstrates that the conserved yeast protein Moh1 links metabolism, gene expression, and cell structure, thereby altering cell envelope permeability and leading to selective stress responses.
Fungistatic effect of sorbic acid on yeast cells via translational repression involving eIF2α phosphorylation and formation of Ded1- and eIF2B-granules
June 17, 2026
The findings of this study provide new insights into the physiological effects of sorbic acid on yeast cells, specifically regarding the regulation of translation-related factors.
Genomic epidemiology of carbapenemase-producing Klebsiella pneumoniae circulating in a Chilean tertiary-care hospital (2021–2022): Molecular characterization, resistance-virulence convergence, and clinical associations
May 21, 2026
This study characterized carbapenem-resistant Kp (CR-Kp) strains isolated at the Hospital Clínico Universidad de Chile (HCUCH) 2021-2022 and explored associations with clinical characteristics.