Understanding the pathogenesis of infectious diseases by single-cell RNA sequencing
Authors:Wanqiu Huang1, Danni Wang1 and Yu-Feng Yao1,2
doi: 10.15698/mic2021.09.759
Volume 8, pp. 208 to 222, published 04/08/2021.
1 Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
2 Department of Infectious Diseases, Shanghai Ruijin Hospital, Shanghai 200025, China.
Keywords:
single-cell RNA sequencing, infectious diseases, bacteria, viruses, fungi, parasites, immune response.
Corresponding Author(s):
Conflict of interest statement:
The authors have declared no conflict of interest.
Please cite this article as:
Wanqiu Huang, Danni Wang and Yu-Feng Yao (2021). Understanding the pathogenesis of infectious diseases by single-cell RNA sequencing. Microbial Cell 8(9): 208-222. doi: 10.15698/mic2021.09.759
© 2021 Huang et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduc-tion in any medium, provided the original author and source are acknowledged.
Abstract:
Infections are highly orchestrated and dynamic processes, which involve both pathogen and host. Transcriptional profiling at the single-cell level enables the analysis of cell diversity, heterogeneity of the immune response, and detailed molecular mechanisms underlying infectious diseases caused by bacteria, viruses, fungi, and parasites. Herein, we highlight recent remarkable advances in single-cell RNA sequencing (scRNA-seq) technologies and their applications in the investigation of host-pathogen interactions, current challenges and potential prospects for disease treatment are discussed as well. We propose that with the aid of scRNA-seq, the mechanism of infectious diseases will be further revealed thus inspiring the development of novel interventions and therapies.