Mechanisms underlying lactic acid tolerance and its influence on lactic acid production in Saccharomyces cerevisiae

Authors:

Arne Peetermans1,2, María R. Foulquié-Moreno1,2 and Johan M. Thevelein1,2,3

doi: 10.15698/mic2021.06.751
Volume 8, pp. 111 to 130, published 14/04/2021.

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Affiliations:

1 Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Flanders, Belgium.

2 Center for Microbiology, VIB, Kasteelpark Arenberg 31, B-3001, Leuven-Heverlee, Flanders, Belgium.

3 NovelYeast bv, Open Bio-Incubator, Erasmus High School, Laarbeeklaan 121, 1090 Brussels (Jette), Belgium.

Keywords: 

lactic acid tolerance, Saccharomyces cerevisiae, lactic acid production, yeast cell factory, lactic acid stress response.

Corresponding Author(s):

Johan Thevelein, Telephone: +32 - 468 21 00 12; johan.thevelein@novelyeast.com

Conflict of interest statement:

The authors report no competing interests.

Please cite this article as:

Arne Peetermans, María R. Foulquié-Moreno and Johan M. Thevelein (2021). Mechanisms underlying lactic acid tolerance and its influence on lactic acid production in Saccharomyces cerevisiae. Microbial Cell 8(6): 111-130. doi: 10.15698/mic2021.06.751

© 2021 Peetermans et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduc-tion in any medium, provided the original author and source are acknowledged.

Abstract:

One of the major bottlenecks in lactic acid production using microbial fermentation is the detrimental influence lactic acid accumulation poses on the lactic acid producing cells. The accumulation of lactic acid results in many negative effects on the cell such as intracellular acidification, anion accumulation, membrane perturbation, disturbed amino acid trafficking, increased turgor pressure, ATP depletion, ROS accumulation, metabolic dysregulation and metal chelation. In this review, the manner in which Saccharomyces cerevisiae deals with these issues will be discussed extensively not only for lactic acid as a singular stress factor but also in combination with other stresses. In addition, different methods to improve lactic acid tolerance in S. cerevisiae using targeted and non-targeted engineering methods will be discussed.