Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae
Authors:António Rego#, Ana Marta Duarte#, Flávio Azevedo#, Maria João Sousa, Manuela Côrte-Real and Susana R. Chaves
Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
# António Rego, Ana Marta Duarte and Flávio Azevedo contributed equally to this work.
Keywords:
yeast, apoptosis, acetic acid, MAPK, CWI.
Abbreviations: PCD - programmed cell death, CRC - colorectal carcinoma, CWI - cell wall integrity, HOG - High Osmolarity Glycerol, MOMP - mitochondrial outer, membrane permeabilization, PKC - Protein kinase C, MAPKKK - Mitogen Activated Protein kinase kinase kinase, MAPKK - MAP kinase kinase, MAPK - MAP kinase, ROS - reactive oxygen species, ECM - extracellular matrix, PI - propidium iodide, DHE - dihydroethidium, CFU - colony-forming units.
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Conflict of interest statement:
The authors declare no conflict of interest.
Please cite this article as:
António Rego, Ana Marta Duarte, Flávio Azevedo, Maria João Sousa, Manuela Côrte-Real and Susana R. Chaves (2014). Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae. Microbial Cell 1(9): 303-314.
© 2014 Rego et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
Abstract:
Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, the cell wall integrity pathways were the major mediators, especially the latter, which we characterized further. Screening downstream effectors of this pathway, namely targets of the transcription factor Rlm1p, highlighted decreased cell wall remodeling as particularly important for acetic acid resistance. Modulation of cell surface dynamics therefore emerges as a powerful strategy to increase acetic acid resistance, with potential application in industrial fermentations using yeast, and in biomedicine to exploit the higher sensitivity of colorectal carcinoma cells to apoptosis induced by acetate produced by intestinal propionibacteria.
doi: 10.15698/mic2014.09.164
Volume 1, pp. 303 to 314, published 27/08/2014.