Salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA

Authors:

Qiuqiang Gao1, Liang-Chun Liou2, Qun Ren2, Xiaoming Bao3 and Zhaojie Zhang2
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Affiliations:

1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.

2 Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.

3 State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.

Keywords: 

cell wall damage; reactive oxygen species; SCW11; salt stress; Saccharomyces cerevisiae.

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Corresponding Author(s):

Dr. Zhaojie Zhang, Department of Zoology and Physiology, University of Wyoming; Laramie, WY, 82071, USA zzhang@uwyo.edu

Conflict of interest statement:

The authors declare no conflict of interest.

Please cite this article as:

Qiuqiang Gao, Liang-Chun Liou, Qun Ren, Xiaoming Bao and Zhaojie Zhang (2014). Salt stress causes cell wall damages in yeast cells lacking mitochondrial DNA. Microbial Cell 1(3): 94-99.

© 2014 Gao et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.

Abstract:

The yeast cell wall plays an important role in maintaining cell morphology, cell integrity and response to environmental stresses. Here, we report that salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA (ρ0). Upon salt treatment, the cell wall is thickened, broken and becomes more sensitive to the cell wall-perturbing agent sodium dodecyl sulfate (SDS). Also, SCW11 mRNA levels are elevated in ρ0 cells. Deletion of SCW11 significantly decreases the sensitivity of ρ0 cells to SDS after salt treatment, while overexpression of SCW11 results in higher sensitivity. In addition, salt stress in ρ0 cells induces high levels of reactive oxygen species (ROS), which further damages the cell wall, causing cells to become more sensitive towards the cell wall-perturbing agent.

doi: 10.15698/mic2014.01.131
Volume 1, pp. 94 to 99, published 03/03/2014.

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