Yeast quiescence exit swiftness is influenced by cell volume and chronological age

Authors:

Damien Laporte1, Laure Jimenez1, Laëtitia Gouleme1, Isabelle Sagot1

doi: 10.15698/mic2018.02.615
Volume 5, pp. 104 to 111, published 06/12/2017.

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Affiliations:

1 CNRS, Université de Bordeaux – Institut de Biochimie et Génétique Cellulaires, UMR5095 – 33077 Bordeaux cedex, France.

Keywords: 

S. cerevisiae, quiescence, aging, cell size

Corresponding Author(s):

Isabelle Sagot, , Institut de Biochimie et Génétique Cellulaires - Unité Mixte de Recherche 5095, Centre National de la Recherche Scientifique, Université de Bordeaux - CS61390, F-33077 Bordeaux Cedex, France; Tel: +33 5 56 99 90 21; Fax: +33 5 56 99 90 55; isabelle.sagot@ibgc.cnrs.fr

Conflict of interest statement:

The authors certify that they have NO affiliations with or involvement in any organization or entity with any financial or non-financial interest, in the subject matter or materials discussed in this manuscript.

Please cite this article as:

Damien Laporte, Laure Jimenez, Laëtitia Gouleme, Isabelle Sagot (2017). Yeast quiescence exit swiftness is influenced by cell colume and chronological age. Microbial Cell 5(2): 104-111. doi: 10.15698/mic2018.02.615

© 2017 Laporte et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.

Abstract:

Quiescence exit swiftness is crucial not only for micro-organisms in competition for an environmental niche, such as yeast, but also for the maintenance of tissue homeostasis in multicellular species. Here we explore the effect of replicative and chronological age on Saccharomyces cerevisiae quiescence exit efficiency. Our study reveals that this step strongly relies on the cell volume in quiescence but is not influenced by cell replicative age, at least for cells that have undergone less than 10 divisions. Furthermore, we establish that chronological age strongly impinges on cell’s capacities to exit quiescence. This effect is not related to cell volume or due to cell’s inability to metabolize external glucose but rather seems to depend on intracellular trehalose concentration. Overall, our data illustrate that the quiescent state is a continuum evolving with time, early and deep quiescence being distinguishable by the cell’s proficiency to re-enter the proliferation cycle.