Untargeted metabolomics confirms and extends the understanding of the impact of aminoimidazole carboxamide ribotide (AICAR) in the metabolic network of Salmonella enterica
November 22, 2017
In Salmonella enterica, aminoimidazole carboxamide ribotide (AICAR) is a purine biosynthetic intermediate and a substrate of the AICAR transformylase/IMP cyclohydrolase (PurH) enzyme. Data herein describe the use of metabolomics to identify the metabolic state of mutant strains and probe the underlying mechanisms used by AICAR to inhibit thiamine synthesis. The results obtained provide a cautionary tale of using metabolite concentrations as the only data to define the physiological state of a bacterial cell.
Metabolic network structure and function in bacteria goes beyond conserved enzyme components
April 14, 2016
This article comments on work published by Bazurto et al. (MBio, 2016), which demonstrated that conservation of metabolic components was not sufficient to predict network structure and function Escherichia coli.