Back to article: A novel system to monitor mitochondrial translation in yeast
FIGURE 1: Integration of sfGFPm into the mitochondrial genome. (A) Strain construction strategy. (B) Schematic representation of integration of the reporter into the mitochondrial genome via homologous recombination. 5’ and 3’UTR of COX2 drive the expression of sfGFPm. (C) Control of successful expression of sfGFPm via Western blotting using a GFP antibody. 3-phosphoglycerate kinase (Pgk1) served as a loading control.