Research Articles:

Microbial Cell, Vol. 5, No. 3, pp. 158 - 164; doi: 10.15698/mic2018.03.621

A novel system to monitor mitochondrial translation in yeast

Tamara Suhm1, Lukas Habernig2, Magdalena Rzepka1, Jayasankar Mohanakrishnan Kaimal3, Claes Andréasson3, Sabrina Büttner2,3 and Martin Ott1

Download PDF download pdf
Show/hide additional information

    1 Department of Biochemistry and Biophysics, Stockholm University, SE-10691 Stockholm, Sweden.

    2 Institute of Molecular Biosciences, University of Graz, A-8010 Graz, Austria.

    3 Department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.

Keywords: mitochondrial translation, flow cytometry, superfolder GFP, strain engineering.

Abbreviations:

CAP – chloramphenicol,

OXPHOS – oxidative phosphorylation,

sfGFP – superfolder GFP,

UTR – untranslated region.
Received originally: 13/10/2017 Received in revised form: 22/12/2017
Accepted: 02/01/2018 Published: 13/01/2018

Correspondence:
Martin Ott, Department of Biochemistry and Biophysics, Stockholm University, SE-10691 Stockholm, Sweden; martin.ott@dbb.su.se

Conflict of interest statement: The authors declare no conflict of interest.
Please cite this article as: Tamara Suhm, Lukas Habernig, Magdalena Rzepka, Jayasankar Mohanakrishnan Kaimal, Claes Andréasson, Sabrina Büttner and Martin Ott (2018). A novel system to monitor mitochondrial translation in yeast. Microbial Cell 5(3): 158-164. doi: 10.15698/mic2018.03.621

Abstract

The mitochondrial genome is responsible for the production of a handful of polypeptides that are core subunits of the membrane-bound oxidative phosphorylation system. Until now the mechanistic studies of mitochondrial protein synthesis inside cells have been conducted with inhibition of cytoplasmic protein synthesis to reduce the background of nuclear gene expression with the undesired consequence of major disturbances of cellular signaling cascades. Here we have generated a system that allows direct monitoring of mitochondrial translation in unperturbed cells. A recoded gene for superfolder GFP was inserted into the yeast (Saccharomyces cerevisiae) mitochondrial genome and enabled the detection of translation through fluorescence microscopy and flow cytometry in functional mitochondria. This novel tool allows the investigation of the function and regulation of mitochondrial translation during stress signaling, aging and mitochondrial biogenesis.

INTRODUCTION

In Saccharomyces cerevisiae the mitochondrial genome encodes eight proteins of which seven are membrane proteins and core subunits of the oxidative phosphorylation system (OXPHOS) [1]. Respiratory activity, therefore, depends on mitochondrial translation, but the molecular mechanisms, regulation and timing of mitochondrial translation are largely unexplored [2]. A major obstacle when analyzing mitochondrial translation in cells is the need to inhibit cytosolic translation in order to follow incorporation of radiolabeled amino acids into mitochondrial translation products [3]. Such pharmacological inhibition impacts severely on cellular homeostasis and alters signaling cascades. For example, inhibition of cytosolic translation using cycloheximide leads to an increase in free amino acid levels, which in turn activates TOR signaling [4][5][6] and thus affects cell growth, nutrient signaling and life span [7][8][9]. Additionally, cycloheximide treatment alters protein degradation [10] and acutely impacts on mitochondrial translation within very short time frames [11]. To directly monitor mitochondrial protein synthesis without interfering with cellular protein homeostasis new experimental tools are needed.

Employing biolistic transformation we have integrated a gene encoding superfolder GFP into the mitochondrial genome. This reporter is compatible with mitochondrial respiratory function and enables the direct detection of mitochondrial translation in vivo as GFP fluorescence. This novel tool will facilitate future studies on the regulation and timing of mitochondrial translation.

RESULTS AND DISCUSSION

Integration of a superfolder GFP gene into the mitochondrial genome

In a pioneering previous study, a gene encoding fluorescence-enhanced GFP was inserted into the mitochondrial genome to replace the open reading frame of COX3 [12]. The resulting strain was respiration deficient and expressed only weak fluorescence, which limited its usefulness in fluorescence microscopic and flow cytometric experiments. This limitation could be explained by poor folding of GFP expressed in the context of the mitochondrial translation system [13], which is specialized on the production of membrane proteins. To circumvent the folding problem, we employed superfolder GFP (sfGFP), which folds with enhanced kinetics resulting in a more stable protein [14]. To allow translation of the mRNA by mitochondrial ribosomes its coding sequence was flanked by the authentic 5’ and 3’ untranslated regions (UTR) of COX2 [15][16][17][18], coding for the cytochrome oxidase subunit Cox2. To avoid the respiratory deficiency associated with inactivation of mitochondrial genes, we engineered a new mitochondrial genome that coded for sfGFP as an additional ninth open reading frame. The final engineered gene was termed sfGFPm and was cloned into the pPT24* plasmid (Fig 1A-B), yielding a plasmid (pPT24*-sfGFPm) that contained COX2 5’UTR-sfGFPmCOX2 3’UTR as well as the authentic COX2, including its upstream sequences (Fig 1B). pPT24*-sfGFPm was delivered into a strain that lacks mtDNA (rho0) using biolistic transformation [19]. After selection, the resulting strain that carried pPT24*-sfGFPm was used to cytoduce strain cox2-62, which lacks a functional COX2 gene, to gain respiratory competence by homologous recombination of the two mitochondrial genomes [20]. Finally, we transferred the novel mitochondrial genome into the strain W303 resulting in the strain sfGFPm. Western blot analysis demonstrated that this engineered genome expressed readily detectable levels of sfGFPm (Fig 1C).

FIGURE 1: Integration of sfGFPm into the mitochondrial genome. (A) Strain construction strategy. (B) Schematic representation of integration of the reporter into the mitochondrial genome via homologous recombination. 5’ and 3’UTR of COX2 drive the expression of sfGFPm. (C) Control of successful expression of sfGFPm via Western blotting using a GFP antibody. 3-phosphoglycerate kinase (Pgk1) served as a loading control.

Expression of mitochondrially encoded sfGFPm does not disturb mitochondrial function

We assessed the impact of sfGFPm expression on mitochondrial function by monitoring respiratory growth on non-fermentable medium and found that it was similar between wild type and the sfGFPm strain (Fig 2A and 2B). As predicted, steady state levels of respiratory chain subunits were unchanged and showed the expected increase when cells were grown in non-fermentable compared to fermentable medium (Fig 2C). Finally, we checked for respiratory chain assembly into supercomplexes as well as cytochrome oxidase activity and found that both were unchanged compared to wild type (Fig 2D). We therefore conclude that expression of sfGFPm does not alter the assembly of respiratory complexes or the general respiratory competence of the cells.

FIGURE 2: Expression of sfGFPm does not disturb mitochondrial func-tion. (A) Wild type (WT) and sfGFPm were streaked out on fermentable (Glucose) and non-fermentable (Glycerol) medium. (B) Doubling time during exponential growth phase in glucose (Glc) and glycerol (Gly). Data represent the mean of three independent experiments +/- SD. (C) Steady state levels of OXPHOS subunits in WT and sfGFPm during exponential phase in glucose (Glc), galactose (Gal) or glycerol (Gly). Whole cell extracts were separated on SDS-PAGE and analyzed with Western Blotting using the indicated antibodies. (D) Isolated mitochondria of WT and sfGFPm were lysed in digitonin and protein complexes were separated by blue-native PAGE. Supercomplexes (III2IV and III2IV2) were either visualized by Coomassie staining or complex IV in-gel activity assay.



Expression of sfGFPm followed by flow cytometry

We next explored the possibility to use sfGFPm as an optical readout for mitochondrial translation. First we checked the steady-state levels of sfGFPm by Western blotting during respiration and fermentation to verify that mitochondrially encoded sfGFP follows the same expression pattern as other mitochondrially encoded proteins. As expected sfGFPm protein levels increased in the presence of galactose and glycerol after 6 and 8 hours in the same way as Cox2 levels (Fig 3A-B). Next we determined GFP fluorescence by flow cytometry under the same conditions and observed an increase in the fluorescent signal when cells were grown for 6, 8 and 10 hours in galactose or glycerol containing medium (Fig 3C-D). To control for mitochondrial biogenesis we used mCherry-tagged Cit1, a nuclear encoded protein that is imported into mitochondria from the cytosol [21]. Similar to sfGFPm, Cit1-mCherry levels also increased when grown in the presence of galactose or glycerol, indicating a general increase in mitochondrial biogenesis induced by galactose and glycerol (Fig 3C-D). In sum, these results confirm that sfGFPm represents a functional reporter for the analysis of mitochondrial translation in vivo without the need to inhibit cytosolic translation. Flow cytometry as quantitative readout opens up many possibilities to interrogate mitochondrial translation with single cell resolution and allows the combination of sfGFPm with additional fluorophores for a multitude of simultaneous analyses.

FIGURE 3: Analysis of sfGFPm expression. (A) Cells from an overnight culture in YPD were grown to exponential phase for six or eight hours on different carbon sources. Whole cell extracts were separated on SDS-PAGE and analyzed with Western Blotting using the indicated antibodies. (B) Quantification of Western blotting signal of three independent experiments represented as means +/- SD. GFP signals were normalized to that of 3-phosphoglycerate kinase (Pgk1) and the YPD signal was set to 1. (C) Flow cytometry histogram showing sfGFPm and Cit1-mCherry signals of cells grown on the indicated carbon sources. (D) As in A) but cells were analyzed using flow cytometry. Signal intensities of sfGFPm and Cit1-mCherry were quantified from eight independent samples, and the fluorescence intensity recorded for a strain lacking any fluorescent tag was subtracted as background. Signals on galactose or glycerol were normalized to the respective time point on glucose and the mean +/- SEM is depicted.

sfGFPm allows detection of changes in mitochondrial gene expression

We determined the stability of sfGFPm by inhibiting mitochondrial translation using chloramphenicol (CAP). Using immunoblotting coupled with detection by near-infrared fluorescence, we found that sfGFPm levels declined over four hours after treatment with CAP (Fig 4A). Accordingly, flow cytometry confirmed that already two hours after block of mitochondrial translation via CAP, a decrease in GFP intensity was detectable. While this loss of sfGFPm signal became even more evident over time (Fig 4B), the nuclear encoded mitochondrial protein Cit1-mCherry was stable (Fig 4B). To further test the reporter construct, we made use of glucose repression of mitochondrial function [22][23][24]. When adding glucose to non-fermentable medium, mitochondrial biogenesis as well as translation and OXPHOS assembly are repressed. During glucose repression, we observed a pronounced decline in sfGFPm levels by Western blotting and flow cytometric evaluation. As expected, Cit1-mCherry levels were also decreased by glucose-induced repression of mitochondrial biogenesis (Fig 4C-D). Taken together, both western blot analysis and flow cytometric evaluation of sfGFPm faithfully report the specific inhibition of mitochondrial translation by CAP as well as the general down regulation of mitochondrial biogenesis during glucose repression.

FIGURE 4: Impact on sfGFPm protein levels by inhibition of mitochondrial translation or glucose repression. (A) Cells from an overnight culture in YPD were grown to exponential phase for six hours in glycerol. Cells were treated with 2 mg/ml chloramphenicol (CAP) or vehicle control (VC) for the indicated times and whole cell extracts were separated on SDS-PAGE and analyzed with Western Blotting using the indicated antibodies. The fluorescence signals of 6 independent experiments were quantified and the GFP signals were normalized to the tubulin signals. Data is depicted as fold of the respective untreated time point and the mean +/- SEM is displayed. (B) As in (A) but cells were analyzed using flow cytometry. Signals of sfGFPm and Cit1-mCherry were quantified from eight independent samples, and the fluorescence intensity recorded for a strain lacking any fluorescent tag was subtracted as background before signals from treated cells were normalized to control values. Numbers indicate significance values from student t-test. (C) Cells were grown as in A, but exposed to glucose. (D) As in (B) but cells were exposed to glucose. Numbers indicate significance values from student t-test.

sfGFPm visualized by fluorescence microscopy

Finally, we visualized sfGFPm via fluorescence microscopy. Cells grown in non-fermentable medium in exponential phase showed mitochondrially localized GFP fluorescence (Fig 5), as evidenced by co-localization with Cit1-mCherry (Supplemental movie). This opens the possibility to employ sfGFPm not only as a reporter for mitochondrial translation but also to follow mitochondrial movement during cell division, mitophagy, or mitochondrial biogenesis.

FIGURE 5: sfGFPm visualized by fluorescence microscopy. Cells were grown to exponential phase in non-fermentable medium and sfGFPm and Cit1-mCherry were visualized via fluorescence microscopy.

In conclusion, we have established a functionally neutral mitochondrially encoded GFP reporter, termed sfGFPm, which is easily monitored via Western blotting, flow cytometry and microscopy. Our reporter allows studying mitochondrial translation in vivo without poising cytoplasmic translation and therefore enables detailed studies of mitochondrial function without perturbing cell physiology. The versatile nature of the new mitochondrial genome described here opens up new venues for the investigation of mitochondrial gene expression by optical methods in respiring cells.

MATERIALS AND METHODS

Generation of sfGFPm

A gene encoding superfolder GFP (sfGFP) was was recoded to match mitochondrial codon usage, synthesized (Invitrogen, Life technologies, GeneArt) and cloned into the pKM vector using NdeI and XhoI. pKM contains the 5’ and 3’UTR of COX2 between two EcoRI restriction sites. sfGFPm flanked by the 5’ and 3’UTR of COX2 was then re-cloned from pKM into pPT24* using the EcoRI restriction sites [15][25][26]. pPT24*-sfGFPm contains a part of authentic mtDNA, the sfGFPm under control of the 5’ and 3’UTR of COX2 and, downstream of sfGFPm, the full COX2 gene including its 5’ and 3’UTR. pPT24*-sfGFPm was integrated into the mitochondrial genome via biolistic transformation and homologous recombination. Briefly, the rho0 kar1-1 DFS160 strain [27] was grown in YP medium containing 2% raffinose and spread on SD-Leu plates containing sorbitol (1 M). The nuclear marker plasmid pRS315 (5 µg), as well as the pPT24*-sfGFPm (10-15 µg), were precipitated on tungsten particles (100 µl tungsten particle (0.5 µm), 1 M CaCl2, 16 mM spermidine) and spread on flying discs. The particles were bombarded onto a lawn of yeast cells with a particle gun (Biorad, USA). After four to five days positive nuclear transformants were collected on a master plate and crossed to a cox2-62 tester strain [20][28]. The diploids were screened for respiratory growth. This crossing step was repeated twice with positive clones. Eventually the stable synthetic rho strain containing pPT24*-sfGFPm was repopulated with mtDNA via cytoduction with a strain containing the cox2-62 mutation. A haploid clone that regained respiratory competence was identified and used to transfer mitochondrial DNA into W303 rho0 to create strain MOY1355 (Mat α ade2-1 his3-11,15 trp1-1 leu2-3,112 ura3-1, sfGFPm::cox2, COX2). An mCherry-tagged variant of Cit1 was generated by replacing the stop codon of the endogenous open reading frame with a sequence encoding mCherry followed by a TRP1 selection cassette to yield strain MOY1360 (Mat α ade2-1 his3-11,15 trp1-1 leu2-3,112 ura3-1, CIT1-mCherry::TRP1, sfGFPm::cox2, COX2). Cells were grown at 30˚C in 1% yeast extract, 2% peptone medium supplemented with 2% dextrose (YPD), 2% galactose (YPGal) or 2% glycerol (YPG) under shaking. When indicated 2 mg/mL chloramphenicol (dissolved in ethanol) or 150 mg/mL cycloheximide (dissolved in water) were added.

SDS-PAGE and Western Blotting

Proteins were extracted by alkaline lysis with 370 mM NaOH, precipitated with 8.33% TCA and pellets were resuspended in reducing sample buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol, 100 mM DTT) (modified from [29]). Proteins were separated using 16% acrylamide, 0.2% bisacrylamide SDS-PAGE and blotted on nitrocellulose membrane. Western blot signals were quantified using ImageJ. For immunoblot analyses upon treatment with chloramphenicol (CAP) or addition of 2% glucose, cells equivalent of 4 OD600 were harvested at indicated time points. Proteins were extracted by alkaline lysis with 1.8 M NaOH, 7.5% β-mercaptoethanol and precipitated with 27.5% TCA. Pellets were resuspended in 100 µL urea loading buffer (200 mM Tris-HCl pH 6.8, 8 M Urea, 5% SDS, 1 mM EDTA, 0.02% Bromphenolblue, 15 mM DTT) and incubated at 65°C for 10 min. Proteins were separated on 12.5% Tris-glycine SDS-PAGE and transferred onto nitrocellulose membranes. Membranes were blocked for 1 hour with 1% skim milk in TBST (0.05% Tween) and incubated over night with antibodies directed against alpha-tubulin (ab184970) and GFP (Roche 11814460001) in TBST (0.2% Tween + 1% skim milk). Membranes were washed four times with TBST (0.05% Tween) and incubated for 1 hour with the following near-infrared fluorescent secondary antibodies: anti-mouse IRDye 800CW 926-32210 and anti-rabbit IRDye 680RD 926-68071 diluted 1:20000 in TBST (0.2% Tween + 0.01% SDS). Membranes were washed four times with TBST (0.05% Tween), rinsed twice with TBS and signals were analyzed using Odyssey Fc (Li-499 Cor Biosciences, Lincoln, NE) and Image Studio Lite software.

Native-PAGE and complex IV activity

For native-PAGE, isolated mitochondria (100 µg) were solubilized (50 mM BisTris, 25 mM KCl, 1 mM EDTA, 2 mM aminohexanoic acid, 12% glycerol, 1 mM PMSF, 1x complete, 2% digitonin) for 10 minutes on ice. After a clarifying spin, lysates were separated on a native 3-12% Bis-Tris gel (NativePAGE, ThermoFisher). Supercomplexes were visualized by coomassie staining or complex IV activity assay. Briefly, complex IV activity was visualized by adding 2.5 mM 3,3′-Diaminobenzidine dissolved in 0.05 M phosphate buffer pH 7.4, 1 nM catalase, 1 mg/ml cytochrome c and 240 mM sucrose.

Fluorescence microscopy

Cells were grown at 30°C and live images were taken using a Zeiss LSM 800 Airyscan microscope (Carl Zeiss, Jena, Germany) with a Plan-apochromatic 63X/1.4-numerical aperture oil immersion lens. For confocal excitation of GFP, a 488-nm diode laser was set at 10.0%, and emission was detected between wavelengths 492 and 540 nm. For excitation of mCherry, a 561-nm laser line was used at 2.8%, and emission was detected between 565 and 695 nm. A Z-stack of cells was performed using ZEN blue 2.1 software and maximum intensity projections of the images are shown. Image analysis was done by using ImageJ (National Institutes of Health, Bethesda, MD) and Imaris 8.4 software.

Flow cytometry

1×106 cells were harvested via centrifugation. Cells were washed once with water and resuspended in PBS (PBS, 25 mM potassium phosphate; 0.9% NaCl; adjusted to pH 7.2). For quantification of fluorescence intensities using flow cytometry (BD LSR Fortessa), 30,000 cells were recorded and analyzed with the BD FACSDiva software. A W303a strain carrying no fluorescent tag served as background, and respective fluorescence intensities on either YPD, YPGal or YPGly were subtracted as background.

Statistics

Statistical significance was determined using student’s t-test (unpaired, two-tailed, equal variance).

References

  1. P. Borst, and L.A. Grivell, "The mitochondrial genome of yeast.", Cell, 1978. http://www.ncbi.nlm.nih.gov/pubmed/153200
  2. M. Ott, A. Amunts, and A. Brown, "Organization and Regulation of Mitochondrial Protein Synthesis", Annual Review of Biochemistry, vol. 85, pp. 77-101, 2016. http://dx.doi.org/10.1146/annurev-biochem-060815-014334
  3. T.D. Fox, L.S. Folley, J.J. Mulero, T.W. McMullin, P.E. Thorsness, L.O. Hedin, and M.C. Costanzo, "Analysis and manipulation of yeast mitochondrial genes.", Methods in enzymology, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1706458
  4. A. BEUGNET, A.R. TEE, P.M. TAYLOR, and C.G. PROUD, "Regulation of targets of mTOR (mammalian target of rapamycin) signalling by intracellular amino acid availability", Biochemical Journal, vol. 372, pp. 555-566, 2003. http://dx.doi.org/10.1042/BJ20021266
  5. M. Binda, M. Péli-Gulli, G. Bonfils, N. Panchaud, J. Urban, T.W. Sturgill, R. Loewith, and C. De Virgilio, "The Vam6 GEF Controls TORC1 by Activating the EGO Complex", Molecular Cell, vol. 35, pp. 563-573, 2009. http://dx.doi.org/10.1016/j.molcel.2009.06.033
  6. J. Urban, A. Soulard, A. Huber, S. Lippman, D. Mukhopadhyay, O. Deloche, V. Wanke, D. Anrather, G. Ammerer, H. Riezman, J.R. Broach, C. De Virgilio, M.N. Hall, and R. Loewith, "Sch9 Is a Major Target of TORC1 in Saccharomyces cerevisiae", Molecular Cell, vol. 26, pp. 663-674, 2007. http://dx.doi.org/10.1016/j.molcel.2007.04.020
  7. R. Loewith, and M.N. Hall, "Target of Rapamycin (TOR) in Nutrient Signaling and Growth Control", Genetics, vol. 189, pp. 1177-1201, 2011. http://dx.doi.org/10.1534/genetics.111.133363
  8. S. Wullschleger, R. Loewith, and M.N. Hall, "TOR Signaling in Growth and Metabolism", Cell, vol. 124, pp. 471-484, 2006. http://dx.doi.org/10.1016/j.cell.2006.01.016
  9. T. Suhm, and M. Ott, "Mitochondrial translation and cellular stress response", Cell and Tissue Research, vol. 367, pp. 21-31, 2016. http://dx.doi.org/10.1007/s00441-016-2460-4
  10. C. Dai, J. Shi, Y. Chen, K. Iqbal, F. Liu, and C. Gong, "Inhibition of Protein Synthesis Alters Protein Degradation through Activation of Protein Kinase B (AKT)", Journal of Biological Chemistry, vol. 288, pp. 23875-23883, 2013. http://dx.doi.org/10.1074/jbc.M112.445148
  11. M.T. Couvillion, I.C. Soto, G. Shipkovenska, and L.S. Churchman, "Synchronized mitochondrial and cytosolic translation programs", Nature, vol. 533, pp. 499-503, 2016. http://dx.doi.org/10.1038/nature18015
  12. J.S. Cohen, and T.D. Fox, "Expression of green fluorescent protein from a recoded gene inserted into Saccharomyces cerevisiae mitochondrial DNA.", Mitochondrion, 2001. http://www.ncbi.nlm.nih.gov/pubmed/16120277
  13. C.M. Demlow, and T.D. Fox, "Activity of mitochondrially synthesized reporter proteins is lower than that of imported proteins and is increased by lowering cAMP in glucose-grown Saccharomyces cerevisiae cells.", Genetics, 2003. http://www.ncbi.nlm.nih.gov/pubmed/14668357
  14. J. Pédelacq, S. Cabantous, T. Tran, T.C. Terwilliger, and G.S. Waldo, "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotechnology, vol. 24, pp. 79-88, 2005. http://dx.doi.org/10.1038/nbt1172
  15. S. Gruschke, K. Römpler, M. Hildenbeutel, K. Kehrein, I. Kühl, N. Bonnefoy, and M. Ott, "The Cbp3–Cbp6 complex coordinates cytochromebsynthesis withbc1complex assembly in yeast mitochondria", The Journal of Cell Biology, vol. 199, pp. 137-150, 2012. http://dx.doi.org/10.1083/jcb.201206040
  16. M.G. Ding, C.A. Butler, S.A. Saracco, T.D. Fox, F. Godard, J. di Rago, and B.L. Trumpower, "Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and non-functional cytochrome b proteins", Biochimica et Biophysica Acta (BBA) - Bioenergetics, vol. 1777, pp. 1147-1156, 2008. http://dx.doi.org/10.1016/j.bbabio.2008.04.029
  17. X. Perez-Martinez, "Mss51p promotes mitochondrial Cox1p synthesis and interacts with newly synthesized Cox1p", The EMBO Journal, vol. 22, pp. 5951-5961, 2003. http://dx.doi.org/10.1093/emboj/cdg566
  18. M.C. Costanzo, and T.D. Fox, "Control of Mitochondrial Gene Expression in Saccharomyces Cerevisiae", Annual Review of Genetics, vol. 24, pp. 91-108, 1990. http://dx.doi.org/10.1146/annurev.ge.24.120190.000515
  19. N. Bonnefoy, and T.D. Fox, "Directed Alteration of Saccharomyces cerevisiae Mitochondrial DNA by Biolistic Transformation and Homologous Recombination", Methods in Molecular Biology, pp. 153-166, 2007. http://dx.doi.org/10.1007/978-1-59745-365-3_11
  20. N. Bonnefoy, and T.D. Fox, "In vivo analysis of mutated initiation codons in the mitochondrial COX2 gene of Saccharomyces cerevisiae fused to the reporter gene ARG8m reveals lack of downstream reinitiation.", Molecular & general genetics : MGG, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10660064
  21. M. Rosenkrantz, T. Alam, K.S. Kim, B.J. Clark, P.A. Srere, and L.P. Guarente, "Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.", Molecular and cellular biology, 1986. http://www.ncbi.nlm.nih.gov/pubmed/3540614
  22. R.J. Trumbly, "Glucose repression in the yeast Saccharomyces cerevisiae.", Molecular microbiology, 1992. http://www.ncbi.nlm.nih.gov/pubmed/1310793
  23. M. Carlson, "Glucose repression in yeast", Current Opinion in Microbiology, vol. 2, pp. 202-207, 1999. http://dx.doi.org/10.1016/S1369-5274(99)80035-6
  24. H. Ronne, "Glucose repression in fungi.", Trends in genetics : TIG, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7900189
  25. P.E. Thorsness, and T.D. Fox, "Nuclear mutations in Saccharomyces cerevisiae that affect the escape of DNA from mitochondria to the nucleus.", Genetics, 1993. http://www.ncbi.nlm.nih.gov/pubmed/8514129
  26. T.D. Fox, "Five TGA "stop" codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II.", Proceedings of the National Academy of Sciences of the United States of America, 1979. http://www.ncbi.nlm.nih.gov/pubmed/230513
  27. D.F. Steele, C.A. Butler, and T.D. Fox, "Expression of a recoded nuclear gene inserted into yeast mitochondrial DNA is limited by mRNA-specific translational activation.", Proceedings of the National Academy of Sciences of the United States of America, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8643562
  28. H.M. Dunstan, N.S. Green-Willms, and T.D. Fox, "In vivo analysis of Saccharomyces cerevisiae COX2 mRNA 5'-untranslated leader functions in mitochondrial translation initiation and translational activation.", Genetics, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9286670
  29. S. Silve, C. Volland, C. Garnier, R. Jund, M.R. Chevallier, and R. Haguenauer-Tsapis, "Membrane insertion of uracil permease, a polytopic yeast plasma membrane protein.", Molecular and cellular biology, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1846664

SUPPLEMENTAL INFORMATION

Download Supplemental Information Download Supplemental Information

ACKNOWLEDGMENTS

We thank Dr. Tom Fox (Cornell University, USA) and Dr. Alexander Tzagoloff (Columbia University, USA) for advice and material, and the members of our group for stimulating discussions. This work was supported by grants from the Knut and Alice Wallenberg Foundation, the Carl Tryggers Foundation and the Swedish Research Council. MR holds a Marie-Curie trainee grant from the horizon 2020 project remix. Part of this work was supported by the Austrian Science Fund FWF (P27183-B24) to SB.

COPYRIGHT

© 2018

Creative Commons License
A novel system to monitor mitochondrial translation in yeast by Suhm et al. is licensed under a Creative Commons Attribution 4.0 International License.