Back to article: Decreasing cytosolic translation is beneficial to yeast and human Tafazzin-deficient cells
FIGURE 4: Mitochondrial membrane potential. Variations in mitochondrial ΔΨ were moni-tored by fluorescence quenching of Rhodamine 123, using intact, osmotically-protected, mitochondria isolated from WT, taz1Δ, taz1Δ rei1Δ and taz1Δ rpl6bΔ cells grown in CSM containing 0.5% galactose + 2% ethanol at 36°C until a density of 2-3 OD600nm. The additions were 75 μM ADP, 0.5 μg/ml Rhodamine 123, 75 μg/ml mitochondrial proteins (Mito), 10 μl ethanol (EtOH), 2 mM potassium cyanide (KCN), 4 μM CCCP (carbonyl cyanide-m-chlorophenyl hydrazone) and 4 μg/ml oligomycin (oligo). The shown tracings are representative of four experimental trials. The data for WT and taz1Δ strains were reported previously [60].
60. de Taffin de Tilques M, Tribouillard-Tanvier D, Tetaud E, Testet E, di Rago JP, Lasserre JP (2017). Overexpression of mitochondrial oxodicarboxylate carrier (ODC1) preserves oxidative phosphorylation in a yeast model of Barth syndrome. Dis Model Mech 10(4): 439-450. http://dx.doi.org/10.1242/dmm.027540