Back to article: Decreasing cytosolic translation is beneficial to yeast and human Tafazzin-deficient cells


FIGURE 4: Mitochondrial membrane potential. Variations in mitochondrial ΔΨ were moni-tored by fluorescence quenching of Rhodamine 123, using intact, osmotically-protected, mitochondria isolated from WT, taz1Δ, taz1Δ rei1Δ and taz1Δ rpl6bΔ cells grown in CSM containing 0.5% galactose + 2% ethanol at 36°C until a density of 2-3 OD600nm. The additions were 75 μM ADP, 0.5 μg/ml Rhodamine 123, 75 μg/ml mitochondrial proteins (Mito), 10 μl ethanol (EtOH), 2 mM potassium cyanide (KCN), 4 μM CCCP (carbonyl cyanide-m-chlorophenyl hydrazone) and 4 μg/ml oligomycin (oligo). The shown tracings are representative of four experimental trials. The data for WT and taz1Δ strains were reported previously [60].

60. de Taffin de Tilques M, Tribouillard-Tanvier D, Tetaud E, Testet E, di Rago JP, Lasserre JP (2017). Overexpression of mitochondrial oxodicarboxylate carrier (ODC1) preserves oxidative phosphorylation in a yeast model of Barth syndrome. Dis Model Mech 10(4): 439-450. http://dx.doi.org/10.1242/dmm.027540

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close