FIGURE 8: Co-immunoprecipitation assay.

(A) Co-immunoprecipitation of PAP with either AtBI-1 or AtBI-1ΔC. Total proteins isolated from yeast cells induced to express PAP, AtBI-1, AtBI-1ΔC and vector control (VC) for 6 hours were incubated with V5-antibody and immunoprecipitated with protein A-Sepharose beads. Immunoprecipitated proteins were separated on 15 %SDS-PAGE, transferred to nitrocellulose and probed with affinity purified PAP antibody or with V5-antibody.

(B) The total lysate from cells expressing PAP, AtBI-1 and AtBI-1ΔC subjected to SDS PAGE/immunoblot analysis using V5 and PAP-antibody to show the level of expression of both proteins.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close