FIGURE 3: Analysis of sfGFP^m expression. (A) Cells from an overnight culture in YPD were grown to exponential phase for six or eight hours on different carbon sources. Whole cell extracts were separated on SDS-PAGE and analyzed with Western Blotting using the indicated antibodies. (B) Quantification of Western blotting signal of three independent experiments represented as means +/- SD. GFP signals were normalized to that of 3-phosphoglycerate kinase (Pgk1) and the YPD signal was set to 1. (C) Flow cytometry histogram showing sfGFP^m and Cit1-mCherry signals of cells grown on the indicated carbon sources. (D) As in A) but cells were analyzed using flow cytometry. Signal intensities of sfGFP^m and Cit1-mCherry were quantified from eight independent samples, and the fluorescence intensity recorded for a strain lacking any fluorescent tag was subtracted as background. Signals on galactose or glycerol were normalized to the respective time point on glucose and the mean +/- SEM is depicted.