FIGURE 6: CHX improves cell proliferation and viability of human Tafazzin-deficient cells. These experiments used our previously described HeLa cells in which TAZ gene has been knocked down by RNA interference (shTaz1) and two control cell lines, shWT1 and shTaz1R, in which expression of TAZ1 is not inhibited [67]. (A) Growth curves in 200 µL wells inoculated with 5 000 cells. After reaching a plateau, the cells die and detach from their support. S1, S2 and S3 are the slopes of the proliferation state for each cell lines. (B) Relative slopes (1/h) deduced from the proliferation curves shown in panel A. (C) xCELLigence recording of ShTaz1 proliferation in absence or presence of CHX at a concentration of 50 pM. CHX was added (black arrow) after a 24-hour adhesion step. The cultures were inoculated with 5 000 cells in 200 μL wells. The optimal Cell Index values are indicated along the curves as well as the time (in hours) taken to reach the inflexion point. The horizontal red bar stands for the 48 hours lag phase induced by CHX. Four independent experiments with 4 wells for each growth condition have been done (16 wells in total for establishing mean values). the ±SD is directly drawn on the top of the curves (in black for ShTaz1 and in red for SgTaz1 + 50 pM CHX). (D) xCELLigence recording of ShWT1 proliferation in absence or presence of CHX at a concentration of 50 pM. The cultures were inoculated with 5 000 cells in 200 μL wells. CHX was added before the binding of the cell to the substrate in this case in order to avoid the perturbations induced by the injection of CHX along the trace; the ±SD is directly drawn on the top of the curves (in black for ShWT1 and in red for SgTaz1, there is no significant variation).

67. Gonzalvez F, Schug ZT, Houtkooper RH, MacKenzie ED, Brooks DG, Wanders RJ, Petit PX, Vaz FM, Gottlieb (2008). Cardiolipin provides an essential activating platform for caspase-8 on mitochondria. J Cell Biol 183(4): 681-696. http://dx.doi.org/10.1083/jcb.200803129