FIGURE 4: Golgi PI4P controls Pma1 secretion. (A) The wild type Pma1-mCherry strain (QAY559) was transformed with the PI4P-labeling (2XPH-GFP) plasmid. Transformants were grown in SC-URA with 2% glucose to early log phase (first row), and then starved for glucose for 30 minutes (second row) or treated with 5 mM of MnCl₂ for 30 min (third row) and photographed. (B) The P_ERG6–OSH6 Pma1mcherry strain (FTY520) was transformed with the PI4P-labeling (2XPH-GFP) plasmid. Transformants were grown in SC-URA to mid-log phase (first row), and then starved for glucose for 30 minutes (second row), or treated with 5 mM of MnCl₂ (third row). Hollow arrows point to mother-bud neck where the Pma1 signal is not as bright as other PM of its mother cell. Solid arrows point to small or mid-size budded cells with homogenously distributed Pma1 on the PM of the mother cell.