FIGURE 2. (A) Biofilms formed by L. gasseri, C. albicans and C. glabrata during single or co-cultivation in MRS, as detected by scanning electron microscopy. The biofilms were obtained using the same conditions described in materials and methods, with the exception that we have used 5 mL-polystyrene plates instead of the 8 mm plates used in the fluorescence microscopy imaging. These images were obtained after 24 h of cultivation at 37ºC and 25 rpm using a magnification of 2000x to 35000x over the dried biofilms. For the sake of facilitating identification of cells, in the last column the yeast cells were labelled in yellow, while the bacterial cells were labelled in blue. (B) Image obtained at the nanoscale clearly showing the close cell-cell contacts established between L. gasseri and C. glabrata. The image is representative of others also found while examining the imaged field.