FIGURE 5: The IGP ectodomain is required for retention.

(C) Left panel: Turnover kinetics of BiPN-IGP48 (open symbols) and BiPN-IGP40 (closed symbols) was determined by blocking protein synthesis with cycloheximide and detecting residual protein with anti-HA antibodies. Right panel: Turnover is sensitive to inhibition by 10 µM MG-132 (open symbols) or 20 mM NH₄Cl (closed symbols). Results were normalised to 100% at t = 0. The graph represents the mean of two independent experiments, with the standard error of the mean indicated. Numbers to the left of some panels indicate the positions of co-migrated molecular weight standards and are in kDa.

Go to figure 5A, figure 5B.

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