FIGURE 5: The IGP ectodomain is required for retention.

(C) Left panel: Turnover kinetics of BiPN-IGP48 (open symbols) and BiPN-IGP40 (closed symbols) was determined by blocking protein synthesis with cycloheximide and detecting residual protein with anti-HA antibodies. Right panel: Turnover is sensitive to inhibition by 10 µM MG-132 (open symbols) or 20 mM NH4Cl (closed symbols). Results were normalised to 100% at t = 0. The graph represents the mean of two independent experiments, with the standard error of the mean indicated. Numbers to the left of some panels indicate the positions of co-migrated molecular weight standards and are in kDa.

Go to figure 5A, figure 5B.

For figures in higher resolution please refer to PDF version of the article.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.