Back to article: The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose
FIGURE 3: CIT2 mRNA level in raffinose-grown wild-type and mutant strains in exponential phase growth and en route to acetic acid treatment.
(A) CIT2 mRNA levels were measured by real-time PCR in RAF-WT cells and in deletion mutant cells grown in raffinose medium and collected in exponential phase (OD600 0.6-0.7). GLU-WT cells were also analyzed as control. Percentage of CIT2 mRNA levels, normalized to ACT1 mRNA, as compared with that in RAF-WT cells (100%), was reported.
(B) CIT2 mRNA levels were measured by real-time PCR at the indicated time points in wild-type and mutant strains grown in raffinose medium with (AA, red bars) or without (ctrl, grey bars) the supplementation of 80 mM acetic acid. GLU-WT samples were included as controls. Percentage of CIT2 mRNA levels, normalized to ACT1 mRNA, as compared with that in AA-treated RAF-WT cells at 30 min, was reported.