FIGURE 1: Differential formation of stationary phase-specific granules in Q and NQ cells. Yeast strains carrying the GFP-tagged genes were grown in YPD for 5 days to enter stationary phase and then cells were fractionated using the Percoll gradient to isolate the Q (in the lower layer) and NQ cells (in the upper layer). The fluorescence images of total stationary phase cultures (SP) and the fractionated cells (Q and NQ) from the same strain are shown under identical brightness and contrast settings. Log-phase (LP) images were taken from exponential cell cultures whose OD600 values were between 0.4 and 0.6. Scale bar: 5 μm. BF: brightfield. (A) Components of the actin body (Abp1 and Cap1) and stress granule (Ygr250c and Cdc33). (B) Com-ponents of the P-body (Edc3 and Dcp2) and CUPS (Grh1 and Uso1). (C) Components of the proteasome storage granule (Pre6, Pre2, Pup1 and Rpn1). In most strains, the GFP intensity of granule marker proteins appears to be stronger in Q cells, which is probably due to the fact that the genes encoding these marker proteins have a higher mRNA abundance in Q cells [9].

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