FIGURE 2: Expression of mutant Vps1 in yeast. (A) Whole cell extracts were made from vps1 null cells expressing an empty plasmid, wild type or mutant vps1. Following separation by SDS-PAGE, proteins were transferred to PVDF, then probed with anti-Vps1 antibodies. Size markers in kDa are denoted. Lower panel shows ponceau staining of the blot to indicated loading levels in lanes. (B) The effect of vps1 mutants on cell growth was assessed by growth on solid media. Ten-fold serial dilutions of log phase cultures were spotted onto plates, and cells were allowed to grow at the permissive (30°C) or restrictive temperature 37°C) for 48 hours.

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