FIGURE 2: PolyQ-induced toxicity in CLS models of yeast aging is suppressed by HAP4 overexpression-mediated increase in mitochondrial biogenesis.

(A) Endogenous cell respiration during the exponential and stationary phases of growth of non-induced and induced 103Q cultures overexpressing or not HAP4. Bars represent mean ± S.D., n = 3.

(B) Effect of increased mitochondrial biogenesis by HAP4 overexpression on CLS of yeast expressing 103Q from day 0 in the stationary phase. Error bars represent S.E.M. for 3 independent experiments.

(C) PolyQ expression levels at day 1 after β-estradiol induction estimated by flow cytometry quantification of GFP fluorescence.