FIGURE 2:

(A) RT-PCR analysis of ρ⁰ cells showing SCW11 expression upregulated compared to WT. SCW11 expression in ρ⁰ cells was decreased when treated with 0.6 M NaCl for 30 min, but still higher than in WT cells.

(B) Quantitative analysis of the RT-PCR bands from three independent experiments.

(C) and (D) The cell wall gene SCW11 is involved in salt stress-induced cell death in ρ⁰ cells.

(C) When treated with NaCl, SDS, or both, no apparent change is observed in the growth of WT cells when SCW11 is either deleted or overexpressed.

(D) Deletion of SCW11 in ρ⁰ cells (ρ⁰-scw11) increases their resistance to salt; SCW11 overexpression (ρ⁰+SCW11) causes cells to become more sensitive to SDS after NaCl treatment (0.6 M NaCl for 1 hr). The BG1805 empty vector (WT/ρ⁰+vector) was used as a negative control. Cells were spotted on YPG using 4 μl of 1:5 serial dilutions and cultured at 30^οC for 3-4 days.