FIGURE 3: IGP40 and IGP48 are required for normal cell proliferation.

(A) qRT-PCR of control and tetracycline-induced RNAi lines for IGP34, IGP40 and IGP48 24 hours post-induction (n = 3). Right panel shows a Western blot of IGP48 following RNAi induction after 24 hours with β-tubulin as loading control.

(B) Growth curves of control and tetra-cycline-induced RNAi lines for IGP34, IGP40 and IGP48, showing a growth defect within 24 hours post induction. Representative results for one of two clonal cell lines for each construct studied are shown (n = 2) (all subsequent experiments were performed using this cell line). Cultures were diluted daily to maintain cell densities between 105 and 2 × 106 cells/ml, and a cumulative pseudo-growth curve is shown. Counts were carried out in triplicate, error bars represent standard error of the mean.

For figures in higher resolution please refer to PDF version of the article.

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