FIGURE 3: Filamentous C. albicans cells are more resistant than blastospores to caspofungin-induced programmed cell death. Representative confocal scanning laser fluorescence images of wild-type SC5314 C. albicans cells treated with 0.05 μg/ml caspofungin for 3 hours in YPD. Propidium iodide stains dead cells, dihydrorhodamine 123 (DHR123) indicates the presence of reactive oxygen species (ROS), and the FLICA assay stains for cells with activated intracellular caspases. No PI or FLICA positive cells were observed in the no drug controls. Error bars indicate standard deviations for trials with at least three independent cultures, where at least 300 cells were counted for each trial. A single asterisk indicates statistical significance (p < 0.05) as compared to treated controls. Statistical significance was determined with the unpaired Student’s t-test.