FIGURE 3: Analysis of a BIT-translocant over-expressing RAD54.

(A) Scheme of a further rearrangement event following a primary BIT translocation (represented in Fig. 2A). Two almost identical LTRs, one on the right arm of chromosome VIII and another on the right arm of chromosome XII, recombine leading to a new translocation between chromosome VIII and XII (scheme adapted from [65]).

(B) In BIT translocants over-expressing RAD54, many dead cells are present. Live/Dead staining (Molecular Probe), here combined with Calcofluor, shows the presence of dead cells (in yellow).

(C) Abnormal phenotype of dead BIT translocants over-expressing RAD54.

(D) DAPI staining indicates severe nuclei fragmentation when RAD54 is over-expressed in BIT translocants.

65. Tosato V, Sidari S, and Bruschi CV (2013). Bridge-induced chromosome translocation in yeast relies upon a Rad54/Rdh54-dependent, Pol32-independent pathway. PLoS One 8(4): e60926.

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