FIGURE 3: Measurement of iron content.

(A) Iron sensitivity. Equal amounts of serial dilutions of cells from exponentially grown cultures (10⁵, 10⁴, 10³, 10², 10¹) were spotted onto 40 medium without pantothenate supplemented with 5 mM and 7 mM FeS0₄. The growth was scored after 5 days of incubation at 28°C.

(B) Iron level. Cellular iron levels was quantified in cells grown up to early stationary phase in YNB glucose (0,6%) medium. **P < 0.01 (unpaired two-tailed t-test).

(C) PMS/DCPIP (phenazyne methosulfate/dichlorophenolindophenol) reductase and decylubiquinone reductase activities were measured in mitochondria extracted from cells grown exponentially at 28°C in mineral medium (40) plus 0.2% glucose and 2% galactose without pantothenate. The values of the COASY mutant are expressed as percentage of the activities obtained in the wild type strain.