FIGURE 3: PolyQ-induced toxicity in CLS models of yeast aging is minimized by pre-conditioning the cells in respiratory media.

(A) Endogenous cell respiration during the exponential and stationary phases of growth of non-induced and induced 103Q cultures grown in minimum synthetic media containing either the fermentable glucose (WOGLU) or the non-fermentable ethanol-glycerol (WOEG) as the carbon sources. Error bars are S.D., n = 3. *P < 0.05, **P < 0.005.

(B) Effect of growth in WOEG respiratory media on 103Q yeast CLS compared to WOGLU media. S.D <1 for all samples, n = 3.

(C) PolyQ expression levels at day 1 of CLS after β-estradiol induction estimated by flow cytometry quantification of GFP fluorescence.