FIGURE 4: Quantification of chromatin condensation in hyphae of B. cinerea wild type and BcBIR1 strains.

Fungi were grown for 24 h in PDB medium at 22°C after which H2O2 was added to a final concentration of 10 mM. The cultures were incubated for additional 4 h, samples were removed, stained with Hoechst 33342 and then visualized at 1000x magnification. The percent of nuclei with condensed chromatin was scored and calculated manually (white bars), or by the Condensation module of the SCAN© software (black bars). Data are the mean average ± SEM of three samples, each containing at least 200 nuclei (n = 3). Percentage of apoptotic nuclei in untreated cultures was lower than 15% (not shown). Columns and lines not connected with the same letter are statistically different according to two-way ANOVA (P < 0.05) followed by a post-hoc Tukey HSD analysis (P < 0.001).

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.