FIGURE 4: Sensitivity to H2O2. The cells carrying the wild-type COASY or mutant allele were grown up to exponential phase and incubated for 4h at 28°C or 37°C with the addition of 1 mM and 2 mM H2O2.

(A) After the treatment, equal amounts of serial dilutions of cells (105, 104, 103, 102, 101) were spotted onto YP media plus 2% glucose. The growth was scored after 2 days of incubation at 28°C or 37°C.

(B) Viability of wild-type and mutant strains was measured by C.F.U counting after exposure of cell to 2 mM H2O2 for 4h.**P < 0.01 (unpaired two-tailed t-test).

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