FIGURE 4: S. cerevisiae (rho⁺) cells co-expressing Abp140-GFP and the mitochondrial marker MITO-RFP (strain CRY816). Exponentially growing cells were inspected as live cells (A) or after fixation with 3.7 % formaldehyde for 30 minutes (B) (HCHO-fixed). (Glu+) glucose was present in the medium; (Glu-) exponentially growing cells were shifted to glucose-free medium and incubated for 30 minutes before inspection or fixation. Distribution of fluorescent markers is presented after deconvolution and projection of several image layers in the stack (Z-stack) using Xcellence software (Olympus). Bar, 5 µm.