FIGURE 4: PolyQ-induced toxicity in CLS models of yeast aging is markedly suppressed by pre-conditioning the cells in 0.5% glucose-containing media (caloric restriction or CR).

(A) Endogenous cell respiration during the exponential and stationary phases of growth of non-induced and induced 103Q cultures grown in minimum synthetic (WOGLU) media containing either 2% (control) or 0.5% (CR) glucose as the carbon source. Bars represent mean ± S.D., n = 3.

(B) Effect of CR in 103Q yeast CLS. S.D <1 for all samples, n = 3.

(C) PolyQ expression levels at day 1 after β-estradiol induction estimated by flow cytometry quantification of GFP fluorescence.